Illustration of SSMD, z Score, SSMD*, z* Score, and t Statistic for Hit Selection in RNAi High-Throughput Screens
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چکیده
منابع مشابه
GUItars: A GUI Tool for Analysis of High-Throughput RNA Interference Screening Data
BACKGROUND High-throughput RNA interference (RNAi) screening has become a widely used approach to elucidating gene functions. However, analysis and annotation of large data sets generated from these screens has been a challenge for researchers without a programming background. Over the years, numerous data analysis methods were produced for plate quality control and hit selection and implemente...
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RNA interference (RNAi) high-throughput screening (HTS) has been hailed as the 2nd genomics wave following the 1st genomics wave of gene expression microarrays and single-nucleotide polymorphism discovery platforms. Following an RNAi HTS, the authors are interested in identifying short interfering RNA (siRNA) hits with large inhibition/activation effects. For hit selection, the z-score method a...
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In genome-scale RNA interference (RNAi) screens, it is critical to control false positives and false negatives statistically. Traditional statistical methods for controlling false discovery and false nondiscovery rates are inappropriate for hit selection in RNAi screens because the major goal in RNAi screens is to control both the proportion of short interfering RNAs (siRNAs) with a small effec...
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Today we are riding the crest of a wave of converging new neuroscience In the physics of source localization the forward solution is where a source inside a sphere determines the electrical potential on the surface as calculated using Maxwell's 1864 equations. In contrast, the inverse problem is where the sources are unknown and the location of the sources are estimated by measuring the electri...
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RNA interference (RNAi) high-content screening (HCS) enables massive parallel gene silencing and is increasingly being used to reveal novel connections between genes and disease-relevant phenotypes. The application of genome-scale RNAi relies on the development of high quality HCS assays. Strictly standardized mean difference (SSMD), introduced by Zhang et al. [1], provides a possibility for hi...
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ژورنال
عنوان ژورنال: Journal of Biomolecular Screening
سال: 2011
ISSN: 1087-0571,1552-454X
DOI: 10.1177/1087057111405851